Skip Navigation

This Article
Right arrow Full Text (PDF )
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (46)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Tadir, Y.
Right arrow Articles by Berns, M.W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tadir, Y.
Right arrow Articles by Berns, M.W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 6, No. 7, pp. 1011-1016, 1991
© 1991 European Society of Human Reproduction and Embryology

review-article

REVIEW

Micromanipulation of gametes using laser microbeams

Y. Tadir, W.H. Wright, O. Vafa, L.H. Liaw, R. Asch1,2 and M.W. Berns

Beckman Laser Institute and Medical Clinic Departments of Surgery Irvine, CA 92715, USA 2Department of Obstetrics and Gynecology, University of California Irvine, CA 92715, USA

Correspondence: 1To whom correspondence should be addressed

Various microsurgical procedures at the cellular and subcellular levels using laser non-touch techniques are presented and reviewed. In these procedures, the beams of light, varying in their wavelength (range: 14 ns to continuous wave), were directed via microscopes towards the target area. Micromanlpulation of human spermatozoa with a lasergenerated optical trap enabled the assessment of possible effects on sperm motility and measurements of the relative force generated by each single spermatozoon. Furthermore, the optical trap also provides a new approach to the measurements of intracellular forces without physically touching the cell or its organelles, and inducing chromosome movement during cell division is also possible. Laser beams in a specific configuration are able to induce minimal superficial damage to the zona pellucida of oocytes from various species. This manipulation is aimed at increasing the fertilization rate following insemination with low-quality spermatozoa. Another intracellular application of the laser beams is the destniction of extra pronuclei in polyspermic fertilized human oocytes. These procedures require special equipment which is not commonly available. However, simpler devices may be developed if the advantages of this novel technology are demonstrated.

Key words: laser microbeam/micromanipulation/optical trap/polyspermic fertilization/zona drilling


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
T. Ebner, M. Moser, and G. Tews
Possible applications of a non-contact 1.48 {micro}m wavelength diode laser in assisted reproduction technologies
Hum. Reprod. Update, July 1, 2005; 11(4): 425 - 435.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
T. Ebner, C. Yaman, M. Moser, M. Sommergruber, J. Hartl, and G. Tews
Laser assisted immobilization of spermatozoa prior to intracytoplasmic sperm injection in humans
Hum. Reprod., December 1, 2001; 16(12): 2628 - 2631.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
E. Mantoudis, B.T. Podsiadly, A. Gorgy, G. Venkat, and I.L. Craft
A comparison between quarter, partial and total laser assisted hatching in selected infertility patients
Hum. Reprod., October 1, 2001; 16(10): 2182 - 2186.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Ashkin
Optical trapping and manipulation of neutral particles using lasers
PNAS, May 13, 1997; 94(10): 4853 - 4860.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.