Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (26)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Miller, J. E.
Right arrow Articles by Smith, T. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miller, J. E.
Right arrow Articles by Smith, T. T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 16, No. 5, 918-924, May 2001
© 2001 European Society of Human Reproduction and Embryology

The effect of intracytoplasmic sperm injection and semen parameters on blastocyst development in vitro

Jane E. Miller and T. Timothy Smith1,

North Hudson IVF Center, 385 Sylvan Avenue, Englewood Cliffs, NJ 07632, USA

The present study compares the development and quality of blastocysts derived from conventional oocyte insemination with those derived from intracytoplasmic sperm injection (ICSI). Oocytes were collected from patients undergoing ovarian stimulation with human menopausal gonadotrophins for IVF. Patients with normal semen were assigned to conventional oocyte insemination while those with progressive motility <20% and/or normal sperm morphology <=4% were assigned to ICSI. Resulting embryos were cultured for up to 6 days. The mean number and percentage of embryos reaching the blastocyst stage and the mean number and percentage of blastocysts of high quality on days 5–6 were assessed for both treatment groups and compared. The influence of paternal factors (sperm concentration, motility, progressive motility, morphology) on blastocyst development and quality were assessed by regression analyses. Significantly more ICSI-derived embryos arrested at the 5- to 8-cell stage (P = 0.024) concomitant with the activation of the paternal genome than those derived from conventional oocyte insemination. Significantly fewer ICSI-derived embryos reached the blastocyst stage on days 5–6 (P < 0.001) and significantly fewer ICSI-derived embryos were of high quality (P = 0.002) compared with conventional oocyte insemination. When treatment groups were combined and evaluated by regression analysis, progressive motility and sperm morphology were significantly correlated with diminished blastocyst development and quality (P < 0.05). From these data, we conclude that paternal factors and/or performing ICSI in cases of severe male factor infertility may have a detrimental effect on blastocyst development and their quality.

Key words: blastocyst/ICSI/paternal effects/spermatozoa

1 To whom correspondence should be addressed. E-mail: twopn{at}optonline.net


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
T. Ebner, M. Moser, M. Sommergruber, O. Shebl, and G. Tews
Incomplete denudation of oocytes prior to ICSI enhances embryo quality and blastocyst development
Hum. Reprod., November 1, 2006; 21(11): 2972 - 2977.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Berkovitz, F. Eltes, A. Ellenbogen, S. Peer, D. Feldberg, and B. Bartoov
Does the presence of nuclear vacuoles in human sperm selected for ICSI affect pregnancy outcome?
Hum. Reprod., July 1, 2006; 21(7): 1787 - 1790.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. Sifer, T. Sasportes, V. Barraud, C. Poncelet, J. Rudant, R. Porcher, I. Cedrin-Durnerin, B. Martin-Pont, J.N. Hugues, and J.P. Wolf
World Health Organization grade 'a' motility and zona-binding test accurately predict IVF outcome for mild male factor and unexplained infertilities
Hum. Reprod., October 1, 2005; 20(10): 2769 - 2775.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. C.M. Dumoulin, J. G. Derhaag, M. Bras, A. P.A. Van Montfoort, A. D.M. Kester, J. L.H. Evers, J. P.M. Geraedts, and E. Coonen
Growth rate of human preimplantation embryos is sex dependent after ICSI but not after IVF
Hum. Reprod., February 1, 2005; 20(2): 484 - 491.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. Archer, D. A. Gook, and D. H. Edgar
Blastocyst formation and cell numbers in human frozen-thawed embryos following extended culture
Hum. Reprod., August 1, 2003; 18(8): 1669 - 1673.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
I. Virant-Klun, T. Tomazevic, B. Zorn, L. Bacer-Kermavner, J. Mivsek, and H. Meden-Vrtovec
Blastocyst formation--good indicator of clinical results after ICSI with testicular spermatozoa
Hum. Reprod., May 1, 2003; 18(5): 1070 - 1076.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Plachot, J. Belaisch-Allart, J.-M. Mayenga, A. Chouraqui, L. Tesquier, and A. M. Serkine
Outcome of conventional IVF and ICSI on sibling oocytes in mild male factor infertility
Hum. Reprod., February 1, 2002; 17(2): 362 - 369.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.