Human Reproduction, Vol 13, 3094-3098, Copyright © 1998 by Oxford University Press
WR Edirisinghe, AR Murch, SM Junk and JL Yovich
A man with globozoospermia was treated in our in-vitro fertilization-
intracytoplasmic sperm injection (ICSI) programme. In the treatment cycle,
24 oocytes were collected from his wife. All the oocytes were at metaphase
II stage. The semen sample produced on the day had a normal sperm count,
good motility, but with 100% globozoospermia. All oocytes were injected
with randomly selected spermatozoa and of these, two oocytes showed two
pronuclei and another contained a single pronucleus. The remainder were
unfertilized. The normally fertilized oocytes (two pronuclear) cleaved to
the four-cell stage and were transferred to the patient. At 48 h after
ICSI, the 21 unfertilized oocytes were processed for cytogenetic analysis.
All oocytes contained a haploid chromosome set. The only abnormality seen
was a chromosome fragment in one metaphase. Eighteen oocytes contained
decondensed sperm nuclei and of these, 14 nuclei were beginning to show
signs of premature chromatin condensation (PCC) and the other four showed
strong signs of PCC. Thus it appears that in some forms of globozoospermia,
arrest of nuclear decondensation and/or PCC are another cause of
fertilization failure. The most likely cause for this is the absence or
down-regulation of spermatozoa associated activating factor in round-headed
spermatozoa.
ARTICLES
Cytogenetic analysis of unfertilized oocytes following intracytoplasmic sperm injection using spermatozoa from a globozoospermic man
PIVET Medical Centre, Leederville, Western Australia.
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