Human Reproduction, Vol 12, 224-230, Copyright © 1997 by Oxford University Press
WA van Cappellen, P Kramer, EC van Leeuwen, R de Leeuw and FH de Jong
The objective of this study was to set up a superovulation protocol in
adult cyclic rats by using recombinant human follicle stimulating hormone
(rhFSH; Org32489). Good results were obtained by treatment with decreasing
doses of rhFSH (2.5 to 0.5 IU) during the dioestrus period. The number of
corpora lutea (CL) found in rats treated with this protocol was 43.5 +/-
3.4; this is more than three times the number in saline-treated control
rats (13.0 +/- 0.4). Fertilization of oocytes after superovulation was as
good as after normal ovulation in terms of number of 2-cell stage embryos
found 2 days after mating. The absolute number of implantations was twice
the number observed in saline-treated control rats (23.3 +/- 1.8 versus
10.6 +/- 0.5); therefore the number of implantations per CL was lower in
superovulated rats. The serum concentrations of luteinizing hormone (LH),
endogenous FSH and oestradiol-17beta were decreased during rhFSH treatment,
while the inhibin serum concentration was increased. The progesterone serum
concentration was increased on the days of pro-oestrus and oestrus after
treatment. No difference was observed in the testosterone serum
concentration. Pretreatment with 10 IU rhFSH at oestrus before giving the
decreasing doses of rhFSH during dioestrus reduced the ovulatory response.
Finally, treatment with a constant low dose of rhFSH instead of a
decreasing dose of rhFSH did not result in spontaneous ovulation. However,
ovulation induction by means of a human chorionic gonadotrophin bolus
resulted in superovulation in six out of eight rats. It is concluded that
superovulation in cyclic rats can be achieved using rhFSH treatment.
However, it was found that the type of rhFSH regimen was very important to
achieve appropriate stimulation. The optimal protocol was treatment with
decreasing doses of rhFSH during dioestrus. The oocytes retrieved could be
fertilized as well as oocytes of saline-treated control rats. The results
also indicate that treatment with higher doses of rhFSH might induce a
desensitization for FSH and LH.
ARTICLES
Induction of superovulation in cyclic rats by administration of decreasing doses of recombinant follicle stimulating hormone (Org32489)
Department of Endocrinology & Reproduction, Medical Faculty, Erasmus University, Rotterdam, The Netherlands.
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